A Metabolic Change towards Fermentation Drives Cancer Cachexia in Myotubes

Cachexia is a disorder associated with several pathologies, including cancer. In this paper, we describe how cachexia induced in myotubes by metabolic shift towards fermentation, and the block of modification prevents onset cachectic phenotype. Cachectic myotubes, obtained treatment conditioned medium from murine colon carcinoma cells CT26, show increased glucose uptake, decreased oxygen consumption, altered mitochondria, lactate production. Interestingly, glycolysis 2-deoxy-glucose or dehydrogenase inhibition oxamate induction cachexia, thus suggesting that change greatly involved activation. The induces positive effects also where mitochondrial membrane potential pyruvate activity became similar to control myotubes. Moreover, treated interleukin-6, phenotype fermentative metabolism, features. same results have been achieved treating media human HCT116 pancreatic MIAPaCa-2 cancer cell lines, showing what has observed murine-conditioned wide phenomenon. These findings demonstrate linked formation impedes highlights as possible new tool for counteracting pathology.